Leica SP8 – Zoom Factor Calculator

For Leica SP8 Confocal [1,2], according to Abbe [3] and Nyquist [4,5]
488
488 nm

Zoom Factor = 2.43
The minimum Zoom Factor for the SP8 = 0.75

Z-Stack Step Size = 0.170µm

Field of View = 76µm x 76µm, XY Pixel Size = 74nm x 74nm, Optical section = 754nm
Lateral Resolution = 139nm, Axial Resolution = 529nm

1 Pinhole Effect in Confocal Microscopes, 2 Pinhole Controls Optical Slicing, 3 The Abbe diffraction limit for a microscope, 4 Nyquist–Shannon sampling theorem, 5 The Nyquist rate in Wide Field and Confocal Microscopy, 6 Detectability: A new criterion for evaluation of the confocal microscope
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